Product Description:
Carbohydrate antigen 15-3 (CA15-3) is a epitope fragment MUC-1of breast cancer cell, which is a glycoprotein. CA15-3 present in a variety of cancer, such as the breast, lung cancer and ovarian cancer.
CA15-3 is used for determining breast cancer progression and metastasis, and clinical testing treatment and relapse in Clinical. It has been reported that when CA15-3> 100kU / L, the metastatic breast cancer lesions can be considered. When the treatment is effective about 50% of patients CA15-3 concentrations were significantly decreased; Concentration of CA15-3 are more sensitive than radiological and clinical criteria in 96% local recurrence.
This reagent mainly is used for dynamic monitoring of cancer patients to determine disease progression or auxiliary therapeutic effect, but its concentration is not correlated with tumor size, growth, and the degree of malignancy grading / staging. In some benign diseases (Such as benign breast disease, cirrhosis, hepatitis, etc.) can also be seen that the index increased in varying degrees. Therefore, the CA15-3 should not be considered an effective general screening test for the general population of cancer screening, early diagnosis and other purposes.
Principle:
CA15-3 uses a “sandwich principle”, Enzyme-linked immunological sorbent assay. To measure CA15-3 levels in serum, plastic wells coated with a monoclonal antibodies of CA15-3 are supplied in the kit. The patient’s specimen is added and, during incubation, CA15-3, if present, is fixed to the solid phase antibody, when mono-antibody labelled with HRP is added, creating a HRP-antibody—CA15-3—antibody “sandwich”. After added TMB substrate added, the result is obtained by EIA plate reader.
Related Tips:
- This kit is designated for In-Vitro Diagnostic Use Only.
- Wash procedure. Incomplete washing will adversely affect the test results. Wash each well 3 times with about 0.3ml wash buffer. If no automatic washer is available, washing can be performed manually as follows: Invert the plate vigorously to get all wash buffer out and block the rim of well on absorbent paper for a few seconds. Filling each well with wash buffer and remain 10 seconds. Repeat these steps 3 times. Blot dry the plate by inverting the plate onto absorbent tissue, and striking a hard surface several times.
- Drip procedure. Mix the bottle gently before use. Violent surge may cause too much foam. Invert the bottle and squeeze one or two drop on absorbent tissue to make sure there is no foam. Take the bottle upright the well and make sure the drop does not touch the rim of wells.
- Read procedure. Using the blank well to correct the zero point of reader if single wavelength reader is used. If double wavelength readers with 450nm and 630nm are used, there is no need to correct the zero point.
- Storage. The whole kit should be stored at 2~8℃ for one year. Microplate should be taken to room temperature before opened. This is very important because absorbed atmospheric moisture by cold plates significantly reduces their shelf life. After removing the required number of strips, the plate should be put in the plastic resealable bag with desiccants to minimize exposure to damp air.
- Control serum is prepared with human serum, which is tested negative of HBV, HCV and HIV. But it should still be considered as capable of transmitting viral diseases.
FAQ:
A: The brand name of the Elisa Test Kit product is Biovantion.
Q: What is the model number of the Biovantion Elisa Test Kit?
A: The model number of the Biovantion Elisa Test Kit is BVTE0097.
Q: What is the minimum order quantity for the Biovantion Elisa Test Kit?
A: The minimum order quantity for the Biovantion Elisa Test Kit is 10.
Q: What is the certification of the Biovantion Elisa Test Kit?
A: The Biovantion Elisa Test Kit is certified with ISO13485.
Q: What are the payment terms for the Biovantion Elisa Test Kit?
A: The payment terms for the Biovantion Elisa Test Kit is TT 100% payment.
Q: What is the supply ability for the Biovantion Elisa Test Kit?
A: The supply ability for the Biovantion Elisa Test Kit is 100,000.
Q: What is the price of the Biovantion Elisa Test Kit?
A: The price of the Biovantion Elisa Test Kit is negotiable.
Q: What are the packaging details for the Biovantion Elisa Test Kit?
A: The packaging details for the Biovantion Elisa Test Kit is CARTON.
Q: What is the delivery time for the Biovantion Elisa Test Kit?
A: The delivery time for the Biovantion Elisa Test Kit is 7-15 days.
